Abstract

A method has been developed for the determination of aflatoxins B1 and G1 in laboratory animal food. The procedure involves extraction of the food with acetone and removal of interfering substances from the extract by chromatography on columns of silica gel, followed by thin-layer chromatography on silicic acid. The separated aflatoxins B1 and G1 are estimated spectrophotometrically. Recoveries of added aflatoxins range from 40 to 67% at a sensitivity of 0·03 ppm.

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