Determination of acute toxicity of “Bendamin” drug in laboratory animals

Abstract

The study of acute toxicity is a mandatory stage in the investigation of new drugs, which allows assessing the health of substances for health in the short-term and determining the class of toxicity and breadth of therapeutic action. Therefore, at the first stage of the study, the acute toxicity of Bendamin Cardiomatography was studied, in particular the determination of maximum tolerant, toxic and intermittent lethal doses for laboratory animals. The acute toxicity of Bendamin was determined in two stages: indicative and expanded experiments. In the indicative trial, the drug was administered intragastrically at doses of 50, 500 and 5000 mg/kg body weight. Three white mice and rats were used for each dose. In an expanded experiment, Bendamin was administered intragastrically at doses of 5000 and 10000 mg/kg body weight. In determining the acute toxicity of Bendamin, the DL50 value could not be determined, indicating a low toxicity of the test substance. Thus, the DL50 drug for intragastric administration to white mice is greater than 5000 mg/kg m. The general condition of animals in the studied groups did not differ from the state of intact animals: coordination of movements and skeletal muscle tone, pain response, tactile and acoustic stimuli were adequate, the frequency of breathing and the rhythm of heart rate were within the normal range. At administration of the preparation in a dose of 5000 mg/kg m. T, an insignificant inhibition was established, which is related to the introduction of a large amount of the drug. In determining the coefficients of the mass of the internal organs of laboratory animals, a slight decrease in the weight coefficient of the liver was found in both mice and in rats. The “Bendamin” drug when administered to white mice and rats in the stomach in the maximum amount does not cause symptoms of poisoning and behavioral abnormalities. According to GOST 12.1.007-76, Bendamin, for intragastric administration, according to the degree of hazard belongs to grade 4 toxicity – low toxicity substances (DL50 more than 10000 mg/kg body weight). In the future, it is planned to investigate the chronic toxicity and cumulative properties of the drug in laboratory animals.