Abstract

An enzyme assay for the measurement of total and prostatic acid phosphatase activity by flow injection analysis with electrochemical detection has been developed. The substrate (α-naphthyl phosphate) and enzyme are simultaneously injected to the system by means of a double injection valve. The α-naphtol produced after stopping the flow is amperometrically quantified. The calibration curve is linear from 0.01 to 1.3 u/l with a limit of detection of 0.006 u/l. Results were compared with that obtained spectrophotometrically.

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