Abstract
Avisible and sensitive assay for the quantitative detection of β-glucosidase (β-glu) activity based on Au@CeO2 core-shell nanoparticles (Au@CeO2 NPs)is described. As a hydrolytic enzyme, β-glu can promote the hydrolysis of β-arbutin to hydroquinone (HQ), which can trigger the decomposition of theCeO2 shell. With the single-particle enumeration (SPE) strategy coupled withdark field optical microscopy (DFM), an obvious color alteration of single Au@CeO2 NPs during theetching process can be observed in real-time. By statistically calculating the number of the etched nanoparticles, the β-glu activity level can be quantified accurately. This assay displays a broad linear range from 0.5 to 50mU⋅mL-1 and low detection limit of 0.12mU⋅mL-1. In addition, this method was successfully used to determine β-glu in real samples and acquires satisfactory recoveries in the range of 97.1-102.0%. This study provides a visualization analysis method for β-glu, which may be helpful for monitoring other targets in the future.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
