Electrochemiluminescence immunosensor for highly sensitive detection of 8-hydroxy-2′-deoxyguanosine based on carbon quantum dot coated Au/SiO2 core–shell nanoparticles

Abstract

An electrochemiluminescence (ECL) immunosensor using Pt electrode modified with carbon quantum dot (CQDs) coated Au/SiO2 core–shell nanoparticles was proposed for sensitive detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in this work. Rabbit anti-8-OHdG antibody was covalently bound to CQDs on the surface of Au/SiO2 core–shell nanoparticles. Through signal amplification of Au/SiO2 core–shell nanoparticles, 8-fold enhancement of the ECL signals was achieved. Under optimal conditions, a good linear range from 0.2 to 200ngmL−1 with a low detection limit of 0.085ngmL−1 (S/N=3) for 8-OHdG detection was obtained. Interfering substances tests showed that the corresponding ECL intensity (ΔECL) of 8-OHdG is 8–18 times higher than that of guanine, uric acid (UA) and ascorbic acid, demonstrating its good selectivity for 8-OHdG detection. The ECL immnuosensor exhibits long-term stability with a relative standard deviation (RSD) of 8.5% even after 16 cycles of continuous potential scans. The result of analytical detection of 8-OHdG in real samples was satisfactory. The proposed ECL immnuosensor shows good performance with high sensitivity, specificity, repeatability, stability and provided a powerful tool for 8-OHdG monitoring in clinical samples.