Abstract
An HPLC method for the determination of β-glucan in barley was developed. The β-glucan was hydrolysed with lichenase [ endo-β-(1–3),(1–4)- D-glucan-4-glucanhydrolase from Bacillus subtilis] to oligosaccharides, which were analysed by reversed-phase HPLC using water as the mobile phase at a flow-rate of 0.7 ml/min. The separation of the oligosasaccharides was performed in a C 18 stainless-steel column (Spherisor ODS-2) with 5-μm particles in less than 10 min, with a refractive index detection.
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