Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Wenming Cheng,Xun Zhang,Qiang Song,Weili Lu,Tingni Wu,Qunlin Zhang,Chunru Li

doi:10.1080/21501203.2017.1385546

Wenming Cheng, Xun Zhang + Show 5 more

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https://doi.org/10.1080/21501203.2017.1385546

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Journal: Mycology	Publication Date: Oct 2, 2017
Citations: 14	License type: open-access

Affiliation: Anhui Medical University, Anhui Agricultural University

Abstract

ABSTRACTNucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of Ophiocordyceps and its allies.

Highlights

Pharmacological studies on O. sinensis have revealed that the fungus and its fruiting bodies exerted multiple biological and pharmacological effects, such as immunomodulatory, anti-inflammatory, antioxidant, anti-ageing, antitumour, neuroprotective, hepatoprotective and renoprotective effects, and it has been applied to the treatment of kidney, liver and lung disease (Paterson 2008; Shashidhar et al 2013)
The results showed that the high pressure liquid chromatography (HPLC) method was sensitive, repeatable and accurate for the simultaneous determination of the 13 nucleosides and nucleobases
Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to principal component analysis (PCA)

Summary

Introduction

Pharmacological studies on O. sinensis have revealed that the fungus and its fruiting bodies exerted multiple biological and pharmacological effects, such as immunomodulatory, anti-inflammatory, antioxidant, anti-ageing, antitumour, neuroprotective, hepatoprotective and renoprotective effects, and it has been applied to the treatment of kidney, liver and lung disease (Paterson 2008; Shashidhar et al 2013). Chemical studies on the natural fruiting bodies of Ophiocordyceps sinensis (NFOS) have shown the presence of various carbohydrates, nucleosides, cyclodepsipeptides, alkaloids, sterols, fatty acids, amino acids, vitamins, trace minerals and other components (Lo et al 2013; Zhao et al 2014). Due to its medicinal uses, the non-sustainable collection of NFOS is continuously increasing, and the wild resource is decreasing rapidly. The production of NFOS cultured artificially was reported to be only feasible at laboratory scale so far, and successful cultivation for commercial purpose has not been achieved up till (Li et al 2006a)

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