Abstract
The aim of this study was to determine the ozone concentrations in non-sterile double-distilled water (ABDNE), non-sterile monodistilled water (AMDNE), water for sterile injection (IEA) and 0.9% saline solution (SF 0.9%), using if the DPD method. The ozonized solutions were obtained by micro bubbling the ozone gas for 15 minutes, caused by a low flow of medicinal oxygen (¼L / min), at a concentration of 56mg / L. The determination of ozone concentrations was based on the oxidative staining reaction of N, N′-diethyl-p-phenylenediamine (DPD). EIA presented higher ozone value (9.94 ppm). ABDNE showed concentration in the 5, 10 and 15 minutes of ozonation, respectively, of 6.14 ppm, 7.84 ppm and 8.34 ppm. In the subsequent times, there was instability and alternation of concentration. Ozone measurement tests in SF were performed using the same method, with results of 8.08 ppm, 9.36 ppm and 9.08 ppm, at 5, 10 and 15 minutes, respectively. The EIA proved to be the most suitable for reaching a concentration close to 10 ppm. Studies on ozone dosage and concentrations, as well as on their by-products, should be carried out to support decision-making about safe doses for the application of this therapeutic ozone modality.
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