Abstract
Detergents are widely used in protein chemistry protocols and may be necessary for protein extraction, solubilization and denaturation. However, the presence of detergents is known to interfere with many techniques including Massspectrometry (MS), hence their removal is a pre-requisite for error-free MS analyses. This study evaluates the use of SDR HyperD ® , a unique chromatography resin, for the removal of NP-40, Triton X-100 and CHAPS detergents from protein samples. Additionally, a highly sensitive (detection limit 100 ppm) MS based method for detection and quantitation of these detergents is described. SDR HyperD ® is used in combination with Nanosep ® spin devices format, readily amenable for high-throughput applications. SDR HyperD ® treatment results in substantial improvement in MS signal-intensity of proteins. Estimation of residual detergents in treated samples establishes that SDR
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