Detection Staphylococcus aureus contaminated some door handles and table surfaces and their sensitive to antibiotics

Abstract

The current study's objective was to identify and isolate Staphylococcus spp. germs from a few of Omar Al-Mukhtar University's faculty laboratories. It is regarded as the first investigation carried out at Omar Al-Mukhtar University to assess the level of Staphylococcal bacterial contamination of door handles and table surfaces inside the laboratories. 33 isolates from the College of Science's Botany Department were used in the study, which took place in the department's laboratories during the fall semester of the academic year (2022). After evaluating the expanding colonies and using morphological inspections and biochemical tests to diagnose the isolates, it was showed that 90.9% of the samples from the Botany Department were Staphylococcus and that the percentage of S. aureus reached 36.7% of isolates of Staphylococcus. A biofilm-forming capacity was demonstrated by 80% of the isolated bacteria, of which 29% were S. aureus. In order to ascertain the effectiveness of the antibiotics and which ones the bacteria are resistant to, the sensitivity of thirty S. aureus isolates to hospital-grade antibiotics against infection was tested. The findings revealed a high level of resistance to the majority of antibiotics and a definite resistance to gentamicin. Methicillin-resistant MRSA isolates made up 73.3% of the isolates, whereas vancomycin-resistant VRSA isolates made up 70%.