Abstract
Toxoplasmosis is an important worldwide foodborne zoonotic disease. Infected cattle meats is considered a serous cause of human toxoplasmosis. Here, this study assesses the infection with Toxoplasma gonddi (T. gondii) in cattle using meat juice samples from diaphragmatic muscles collected at the slaughter. An in house indirect enzyme linked immunosorbent assay (ELISA), and commercial latex agglutination test (LAT) followed by immunoblotting were developed on the meat juice (fluids) using tachyzoites of locally isolated T. gondii strain. The comparative analysis of the results of the tested juice samples showed an excellent agreement between the in-house indirect ELISA and LAT test in the positive and negative of meat juice. Relative sensitivity was higher for ELISA on diaphragms fluids random samples 80.39%, for the LAT test was 68.6%. Immune-reactive bands of T. gondii local strain Ag with naturally infected meat juice were 116, 83, 65, 30 and 23 KDa. The obtained results concluded that the development of an effective ELISA test to be used in for detection of toxoplasmosis infection of slaughtered cattle in large-scale would be exactly valuable, since the important role that beef plays in epidemiology of T. gondii, in particular the hazard of transmission to human and food safety.
Highlights
Toxoplasmosis is a world-wide distributed infection is due the infection by the zoonotic Toxoplasma gondii (T. gondii)) protozoal parasite which can be transmitted to humans and other animals; including cattle and it is an important foodborne zoonosis [1,2]
T. gondii IgG in meat juice using enzyme linked immunosorbent assay (ELISA) ELISA was performed to evaluate the success of tachyzoites of local strain of T. gondii antigen to detected toxoplasmosis antibodies IgG in diaphragms meat juice
In this study the in-house indirect ELISA using tachyzoites antigen of local isolated T. gondii is effective and specific test compared with a commercial latex agglutination test (LAT) as the main standard were used for T. gondii antibodies standard in cattle meat
Summary
Toxoplasmosis is a world-wide distributed infection is due the infection by the zoonotic Toxoplasma gondii (T. gondii)) protozoal parasite which can be transmitted to humans and other animals; including cattle and it is an important foodborne zoonosis [1,2]. Different degrees of specificity were found in sheep naturally and or experimentally infected when comparing ELISA dependent on crude or recombinant antigens [8] Whereas Mikaee et al [9] used serological (ELISA) and molecular tools (PCR), to diagnose toxoplasmosis in sheep, showed that the PCR results were essential in the diagnosis of the infected animals more than ELISA techniques. In Egypt, the antibodies against T. gondii prevalence in goat and sheep was high and may refers to the important role of mutton and other meat as transmissible media of human toxoplasmosis due to the habit of eating undercooked grilled meat [11]. Some alternative methods, based on antibody found in meat juice and multiple diagnostic assays are applied for serological investigation for toxoplasmosis, which is commonly made for the meat of different food animals [12]. While livestock in particular cattle are one of the major meat sources in the world, people who eat infected T. gondii undercooked meat or raw cow milk can be expose to the infection [19]
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