Abstract
Silybum marianum considered as an ancient herb promising plants. In this work, fhese study was design to assess total flavonoids, anti-oxidant and HPLC analysis of Silybum marianum plant extract by methanol in vitro. Total flavonoids had been measured in the extract that about 201.6667±1.52753 mg/ml. anti-oxidant activity of methanolic plant extract Silybum marianum in vitro was estimated by assesment of reductive ability, in concentrations that be tested (0.02, 0.04, 0.08, 0.16, 0.32 and 0.64 mg/ml) , the absorbance had been increased significantly , results of HPLC analysis of plant contain (Ascorbic acid ,Gallic acid Catechin, Rutin, Quercetin, Apigeninand Kaemp ferol) in concentration (0.839, 0.0918, 0.628, 2.989, 0.010, 0.0680, 0.228) ppm respectivily.
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