Abstract
The specific binding between Cy5-labeled goat anti-mouse ImmunoglobulinG (IgG) and mouse IgG with a concentration range from 625 to104 µg ml − 1 has been detected successfully by the oblique-incidence reflectivity difference (OI-RD)method in each procedure of microarray fabrication. The experimental data prove that theOI-RD method can be employed not only to distinguish the different concentrations inlabel-free fashion but also to detect the antibody–antigen capture. In addition, thedifferential treatment of the OI-RD signals can decrease the negative influences of glassslide as the microarray upholder. Therefore the OI-RD technique has promisingapplications for the label-free and high-throughput detection of protein microarrays.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
