Abstract
Seventy six swab samples were collected from Otitis media and stool samples from gastroenteritis cases, were cultured on different culture media, gram positive and gram negative bacteria were isolated and identified. The highest prevalence was Staphylococcus aureus (25%) while Salmonella typhi formed the lowest isolate rate (3.9%) other bacterial isolates varied between these two and comparison was done between effect of the air part of Cydonia oblonga (Quince) plant (leaves, seeds, fruits) to determine the highest effective part on the isolated bacteria. As the inhibitory effect of Cydonia oblonga seed, leaves and fruit alcoholic and aqueous extracts on the isolated bacteria were studied. Alcoholic seed extract showed the highest inhibitory activity on two bacterial species Enterobacter aerogenes and Enterococcus faecalis (25 and 22 mm) respectively followed by Aeromona sp. and Pseudomonas aeruginosa (13 and 11 mm) respectively while alcoholic leaves extract showed antibacterial effect on Staphylococcus aureus only (15 mm), alcoholic fruit extract showed its effect on Enterococcus faecalis (12 mm), Klebsiella sp. (11 mm), Salmonella typhi (10 mm), and Pseudomonas aeruginosa (9 mm). the aqueous seed extract showed an effect on Enterococcus faecalis (11 mm), while the aqueous leaves extract showed the highest effect on Staphylococcus aureus (20 mm), followed by Bacillus subtilis (10 mm), and the aqueous fruit extract showed the highest effect on E.coli (10 mm) while on Enterococcus faecalis, Klebsiella sp. and Enterobacter aerogenes was less (9 mm) for all of them. The (MIC) value of the effective extracts revealed that the highest value of alcoholic seed extract on E.faecalis was (25 mgml), while the least (MIC) was (0.39 mgml) for Aeromona sp. with the remaining MIC values ranging between the two.
Highlights
Otitis media is a cavity filled with air located in petrous bone cavity
During the period of study (76) samples were collected from patient infected with Otitis media and gastroenteritis including ear swab and stool samples
Our results showed predominance of Staph.aureus in patient infected with Otitis media which accounts for (25 %) these results are similar to study of (Jwad, 2011) who isolate Staph.aureus at ratio of (24.6 %) and to the result of (Al- Maadidi, 1999) who isolate it at ratio of 23.4 % (Al-Faris et al, 1998) and (Khalil, 1980) confirmed that Staph.aureus constitute the highest isolation rate between other bacterial causes of infection
Summary
Otitis media is a cavity filled with air located in petrous bone cavity. It is one of the most common infections which occur at all ages but is most common in young children. The pathogensis of Otitis media is multifactorial, implicating interaction between the environment, pathogen, local anatomy, and host. Gastroenteritis is an infection or inflammation of the digestive system. Most gastroenteritis are contagious, it is caused by a number of different micro-organisms including bacteria like (Campylobacter, Salmonella, Shigella) or caused by virus (Rotavirus And Norovirus) or parasites (Giardia, Cryptosporidum). The symptoms of infection including diarrhea, vomiting, stomach pain, fever (Meshram et al, 2009; Sulayman and Shareef, 2007; Koneman et al, 1997) It can be caused by toxins produced by bacteria examples (Staph.aureus, Enterobacter cloacae, Clostridium perfringenes). the symptoms of infection including diarrhea, vomiting, stomach pain, fever (Meshram et al, 2009; Sulayman and Shareef, 2007; Koneman et al, 1997)
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