Abstract
Squalene hydroperoxide present in human skin surface lipids was examined by a chemiluminescence high performance liquid chromatography (CL-HPLC) system. A mixture of cytochrome c and luminol served as the hydroperoxide-specific luminescence reagent for post-column detection in reversed phase HPLC. Squalene hydroperoxide was previously detected in skin surface lipids of the human forehead following exposure to the sun. Therefore, in this study, squalene monohydroperoxide was prepared by dye-sensitized photooxidation and its chemical structure was determined by 1H-NMR, 13C-NMR, and FAB-MS. The detection limit of squalene monohydroperoxide in CL-HPLC system was as low as 1 pmol. Skin surface lipids from forehead, scalp, and dandruff were also studied. By exposure to sun light or after washing with shampoo, squalene monohydroperoxide could be detected in nearly all samples examined. Squalene may thus be considered the first target lipid on human skin surface that incurs oxidative stress by sun light exposure, etc.
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