Abstract
The southern rice black-streaked dwarf virus (SRBSDV) is a severe threat to the yield and quality of rice products worldwide. Traditional detection methods for diagnosing SRBSDV infection show several false positives and thus provide inaccurate findings. However, Western blotting (WB) can precisely solve this problem. In this study, P6—a viral RNA-silencing suppressor—was expressed and purified in vitro. Two polyclonal P6 antibodies were obtained and quantified by enzyme-linked immunosorbent assay and WB. Subsequently, WB was performed using the P6 antibodies to identify SRBSDV antigens derived from the suspected rice samples collected from nine districts in Guizhou, China. The assay results showed that Libo, Pingtang, Huishui, Dushan, and Anshun districts had experienced an SRBSDV outbreak. The virus content in the sampled rice tissues was quantified by WB. Our results revealed that SRBSDV mainly accumulated in rice stems rather than rice leaves. Thus, the findings of our study show that the SRBSDV P6 antibody can be used in WB for detecting and monitoring SRBSDV infection in infected rice plants.
Highlights
Southern rice black-streaked dwarf virus (SRBSDV) is a severe threat to both the yield and quality of rice products worldwide (Wang et al, 2019)
P6 was overexpressed at 101 kDa when the final concentration was increased to 0.7 mM IPTG and when the solution was left for 16 h at 25◦C
Rice plants infected by southern rice black-streaked dwarf virus (SRBSDV) have caused a significant economic loss mainly in China and other Asian countries in recent years (Alonso et al, 2019)
Summary
Southern rice black-streaked dwarf virus (SRBSDV) is a severe threat to both the yield and quality of rice products worldwide (Wang et al, 2019). Ten SRBSDV segments encode five putative structural proteins (P2, P3, P4, P8, and P10) and eight putative nonstructural proteins (P1, P5-1, P5-2, P6, P7-1, P7-2, P9-1, and P9-2) (Mao et al, 2013; Wang et al, 2013; Li et al, 2017; Yu et al, 2017). Among these proteins, P6 is a viral RNA-silencing suppressor that may affect its interaction with other viral proteins such as P7-1 and P9-1 (Wang et al, 2011; Jia et al, 2012; Wu et al, 2013). P7-1 and P9-1 were used as targets for detecting SRBSDV infection (Wang et al, 2015; Ran et al, 2018)
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