Abstract
The aim of this study is detection of present sk gene in the different pathogenic bacteria isolates. We collected 22 bacterial isolates from different patients, by ceasinolytic assay we checked the ability of these isolates to produced the streptokinase protein, 12 isolates given positive result in this assay. The plasmid DNA isolated from that strains and used as a template in polymerase chain reaction (PCR) to amplified sk gene by using gene specific primers. From only two E. coli isolates we obtained the 1.3 kb DNA fragment and by agarose gel electrophoresis we determined the DNA fragments size compare with DNA Ladder.
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