Abstract
Background: Widespread use of quinolones are increasing resistance to these antibiotic in Ureaplasma urealyticum. Quinolone resistance occur in U. urealyticum due to Point mutations in DNA topoisomerase and DNA gyrase genes (gyrA, gyrB, parC and parE). The aim of this study were determination of point mutation in clinical isolate by PCR and sequencing methods. Materials and Methods: To investigate the prevalence of quinolone resistance mutations, 30 U. urealyticum positive sample were gathered from pregnant women, referred to obstetrics and gynecology section or prenatal clinic in Beasat Hospital, Sanandaj, Iran. DNA extraction were performed. Point mutation of target genes were done after PCR amplification reaction by sequencing. Results: The results of gene sequencing showed that the substitution of amino acids in codon 83 parC happened in 5 samples. Aspartic acid 82 Asparagine change caused by amino acid substitution D to N happened in 4 cases. The results of the gyrA gene sequencing showed that the amino acid substitution in codon 104 occurred in 2 sample. GUL104LYS amino acid substitution change occurred in 5 samples. Conclusion: Quinolones are most common antibiotics effective in treatment infections caused by a U. urealyticum. Therefore early detection of resistance genes is essential to correct treatment regime to prevent the spread of resistant strains.
Highlights
Ureaplasma spp. are one of the sexually transmitted pathogens that considered etiology agent of urethritis, prostatitis, bacterial vaginosis, cervicitis and pelvic might be led to infertility in men and women [1,2,3,4]
For treatment of infections caused by this bacteria fluoroquinolones are most used antibiotics
The mechanism of fluoroquinolone resistance in Ureaplasma are depend on point mutations in DNA gyrase and topoisomerase IV [4,5,6,7]
Summary
Ureaplasma spp. are one of the sexually transmitted pathogens that considered etiology agent of urethritis, prostatitis, bacterial vaginosis, cervicitis and pelvic might be led to infertility in men and women [1,2,3,4]. For treatment of infections caused by this bacteria fluoroquinolones are most used antibiotics. This antibiotics killing Ureaplasma through the inhibition of DNA replication. The mechanism of fluoroquinolone resistance in Ureaplasma are depend on point mutations in DNA gyrase and topoisomerase IV [4,5,6,7]. We have determined the mutation rate of U. urealyticum to quinolone antibiotics by using PCR and sequencing of GyrA and GyrB subunits of DNA gyrase and ParC, ParE subunits of topoisomerase IV in clinical isolates. Widespread use of quinolones are increasing resistance to these antibiotic in Ureaplasma urealyticum. Quinolone resistance occur in U. urealyticum due to Point mutations in DNA topoisomerase and DNA gyrase genes (gyrA, gyrB, parC and parE). The aim of this study were determination of point mutation in clinical isolate by PCR and sequencing methods
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