Abstract
According to EC regulations, imported material of tuber‐forming Solarium spp. has to be tested for the absence of defined quarantine viruses. In order to allow an efficient and timesaving application of the quarantine inspection procedure, antisera have been developed and scrutinized for their use by the ELISA technique. The viruses concerned were Andean potato latent virus (APLV), Andean potato mottle virus (APMV), arracacha virus B oca strain (AVB‐O), potato virus T (PVT) and tobacco ringspot virus Andean potato calico strain (TRSV‐Ca). The results show that all viruses can be reliably detected by double‐antibody sandwich (DAS) ELISA. The detection limits were in the range ≤ 1–32 ng ml−1. The strain specificity by DAS‐ELISA of the antisera for APLV and APMV was overcome by mixing antisera from the different strains and strain groups, respectively. Strains C and Lm of APMV seemed to be serologically identical. A comparison of the suceptibility of several wild species of tuber‐forming Solanum with that of several cultivars of Solanum tuberosum showed a higher frequency of infection in the wild species.
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