Abstract

This study aims to identify the content of pork gelatin in jelly candy using Fourier Transform Infrared (FTIR) and Polymerase Chain Reaction (PCR) methods. This method provide information to the public in choosing halal and tested food products. By uses a stepwise cluster survey method to obtain a sample then the samples obtained were isolated in gelatin, analyzed using the FTIR spectrophotometer method, and continued with data analysis using PCA (Principal Component Analysis). In addition, DNA detection analysis of pork gelatin was carried out using the PCR method. The results of the study were FTIR spectrum at wavelengths of 1450 – 1300 cm-1, 1543 cm-1, and 2800-3000 cm-1. The classification of gelatin sources in jelly candy with PCA resulted in the proportion value of Principal Component 1 (PC 1) of 39%, the value of the proportion of Principal Component 2 (PC 2) of 31%, the value of the proportion of Principal Component 3 (PC 3) of 14.5% and the cumulative value of PC 1, PC 2, and PC 3 is 84.5%. DNA amplification of jelly candy samples by PCR proved that all jelly candy samples A, B, C, D, and E did not contain pork.

Highlights

  • Halal food products in a country with a Muslim majority population is a major concern

  • The purpose of this study was to identify pork gelatin in jelly candy products in circulation using the Fourier Transform Infrared (FTIR) and Polymerase Chain Reaction (PCR) methods and to determine the source of gelatin used in jelly candy products

  • Meatball samples numbered 13 and pork DNA were amplified in the PCR results, while meatball samples numbered 1-12 were not amplified so it was concluded that samples numbered 112 did not contain pork

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Summary

Introduction

Halal food products in a country with a Muslim majority population is a major concern. Some of the ingredients used in food products such as gelatin, collagen, and fat are synthesized or extracted from mammals such as cattle, fish, and pigs. Products sourced from pork and its derivatives are prohibited for Muslims [1]. The halal lifestyle is sweeping the world, in countries with Muslim-majority populations and in countries with Muslim-minority populations. The demand for halal products is quite large. Halal aspects are very broad, such as goods and/or services related to food, beverages, drugs, cosmetics, chemical products, biological products, genetically engineered products, as well as goods used, used, or utilized by the community [2]

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