Detection of Pork DNA on Commercially Processed Meat Products Using Taqman qPCR for Label Verification

Abstract

Research has been conducted to detect pork DNA in various commercially processed meat products. This study aims to analyze the content of pork DNA in different commercially processed meat products sold online in Jakarta using a Taqman minor groove binder probe combined with a brief profile of a qPCR machine to verify labels. The sample used in this study consisted of 5 types of processed meat products: canned corned, jerky, shredded, meatballs, and smoked meat. A total of 30 samples had no pork labels, and the five others had pork labels. All products were extracted, the concentration and the purity of DNA were measured, and fragmentation DNA and amplification were analyzed using Taqman qPCR. The results showed that the DNA concentration obtained was 14.02 – 176.04 ng/μL, DNA purity was obtained from 1.70 to 1.92, and all genomic DNA was fragmented. There was no amplification in 30 samples without pork labels. Five samples of pork products underwent PCR amplification with a Ct value of 22.96, 20.72, 26.78, 21.96, and 23.16 for canned corned, jerky, shredded pork meatballs, and smoked pork, respectively. The conclusion is that commercially processed meat products sold online in Jakarta comply with labels regulation by Indonesia Food and Drug Authority and consistently maintain the halalness of their products. The novelties in this study tested various commercially processed meat products sold online in Jakarta.