Abstract
Pesticide poisoning is a menace in Indian subcontinent and is one of main reason behind the reported accidental, homicide or suicidal cases in the society. Conventional pesticide detection methods such as Chromatographic, Mass spectrometry, or High-performance liquid chromatography (HPLC) have several constraints including low sensitivity, costly, and more time consumption. These challenges can be overcome with emerging nanotechnology based electrochemical biosensing platform based on acetylcholinesterase (AChE) enzyme inhibition. These biosensors provide advantages to scientific fraternity of forensic laboratories to being fast in operation, portable, cheap, highly sensitive and user-friendly. In the presented work, an electrochemical biosensor for detection of Organophosphorus (OP) pesticides based upon AChE-inhibition is developed that operates in pico-molar range. The titanium oxide and molybdenum disulfide nanomaterials were synthesized and electrodeposited on a screen printed electrode. Further, AChE was immobilized on the modified electrode surface by crosslinking using glutaraldehyde and chitosan. The developed nano-interface was examined by different electrochemical measurements at every fabrication stage. The developed biosensor is expected to show higher stability and good reproducibility for OP pesticide detection. It was successfully validated for low OP pesticide concentration identification in forensic visceral samples and the detection limit of the sensor was calculated as 50 pM.
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