Abstract
Suspended solids from raw sewage, various stages of wastewater treatment, and Milwaukee Harbor water were examined for associated enteroviruses. The solids were sedimented by centrifugation, and enteroviruses associated with them were eluted by homogenization in fetal bovine serum, analyzed quantitatively by plaque assay on Buffalo green monkey kidney cells, and identified by neutralization with Lim Benyesh-Melnick pooled antisera. Viruses were found at concentrations within approximately one order of magnitude in sediments from all samples. Nine different enteroviruses were identified. All three types of poliovirus were detected in terminally treated (chlorinated) sewage, but the temperature-sensitive marker was not detected in the poliovirus strains isolated from chlorinated samples. Together these data indicate that exploitation of the association of viruses with suspended particles leads to a simple rapid means of monitoring virus distribution in sewage and natural waters.
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