Abstract
Agarose isoelectric focusing followed by protein transfer to cellulose nitrate membrane, double-antibody peroxidase labeling, and avidin-biotin amplification was applied for demonstration of oligoclonal IgG bands in unconcentrated cerebrospinal fluid. When adopted on 5 μl specimens containing 125 ng of IgG, we found this method to be a highly reliable alternative to demonstrate presence of oligoclonal IgG in patients with multiple sclerosis and aseptic meningo-encephalitis.
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