Abstract
In this pilot study, a method for the determination of phthalates in honey was developed. The following phthalates are included: diethyl phthalate, di-isobutyl phthalate, di-n-butyl phthalate, bis (2-Ethylhexyl) phthalate, and di- (n-octyl) phthalate. For the preparation of the samples, the method of liquid-liquid extraction with hexane with an ultrasonic bath was used. The analysis of the prepared samples was performed using gas chromatography and a mass detector. The method is reliable, sensitive, and reproducible with a detection limit of 0.28 - 1.38 ug/kg. This paper presents the results of testing samples of honey stored in glass and plastic packaging for three years in order to determine the migration of phthalates. Dimethyl phthalate was not found in the tested samples. Diethyl phthalate in glass samples from glass packaging was below the LOQ, and it ranged from 2.1 to 4.11 µg / kg in the samples of honey in plastic packaging. The values of di-isobutyl phthalate, di-n-butyl phthalate and bis (2-ethylhexyl) phthalate ranged between 5.32 and 22.60 µg / kg respectively;1.32 – 27.80 µg/kg; 4.45-19.19 µg/kg; di-(n-octyl) phthalate and di-methyl phthalate were < LOQ
Highlights
Plastic is increasingly used for food packaging due to the low cost of materials, its potential for thermal sealing, optical properties, and it is suitable for making different shapes and sizes
In order to obtain softer and more flexible plastic products, so-called softeners are added during their production, and phthalates are the subject of this pilot study
Honey samples were randomly collected from two sources: honey samples that were brought to the Scientific Veterinary Institute “Novi Sad” (NIV-NS) in plastic jars and honey samples that were collected from NIV-NS’s bee yard in glass jars
Summary
Plastic is increasingly used for food packaging due to the low cost of materials, its potential for thermal sealing, optical properties, and it is suitable for making different shapes and sizes. Due to these properties, plastic products for food packaging and beverages have surpassed the use of materials such as glass or tinplate. Plastic can be decomposed into compounds harmful to human health. These are divided into organic and inorganic compounds. In order to obtain softer and more flexible plastic products, so-called softeners are added during their production, and phthalates are the subject of this pilot study
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