Detection of Metallo β-lactamase in Klebseilla pneumoniae Isolated From Upper Respiratory Tract of Carrier individuals In Iraq

Abstract

Klebsiella pneumoniae is an a health care challenge over all the world, due to its resistance to many effective antibacterial, particularly those depend as a standard therapeutic options, Klebsiella carbapenemase producing have been emerged in Indian provinces, this phenomenon then have been disseminated in many countries around the world. The most successful β-lactam hydrolyzing enzymes are belonging to the above antibiotic category is New Delhi metallo β-lactamase, presence of NDM is an indication of multi-drug resistance. K.pneumoniae was isolated from Indian employees in Erbil city and from Iraqi people living in Mosul city, who had previously conducted an interventional surgery at hospitals in India.Of a total of 35 K.pneumoniae isolates, 6 KCP strains were discovered by phenotypic analysis using the modified Hudge test. While molecular screening showed 3 KCP as a true positive result. High molecular sensitivity and specificity, based on Gradiant Multiplex PCR, were found in the comparison with classical diagnosis method.On the other hand, according to the Random Amplification Polymorphic DNA, genetic variation has categorized the six KCP isolates into three clusters due to the existence and absence of random amplicons.