Detection of MecA, MecC and Femb Genes by Multiplex Polymerase Chain Reaction

Abstract

Staphylococcus aureus causing wide range of infectious diseases affecting livestock animals as well as human beings. The bacterium is highly potent to acquire antibiotic resistance, and it is considered a very important agent inducing mastitis in dairy farms in Egypt, as well as other parts of the world, methicillin resistant S. aureus (MRSA) is deliberated one of the most significant causative agents which having public health and economic impacts especially in developing countries, so the present study aimed broadly to obtain a snapshot on the genetic characterization of mecA gene which is responsible to induce the resistance against beta lactam antibiotics. The mecA gene sequence obtained through whole genome sequence process from locally isolated S. aureus from well-established Egyptian dairy farms as well as small scale raising ones. As a control measures, the femB gene was introduced in the multiplex PCR to avoid any contaminants from other staphylococci, also, the mecC gene which is discovered recently as a homologue of mecA gene; was investigated but we did not find any isolates having this homologue.