Identification of methicillin resistant staphylococcus aureus (mrsa) using cefoxitin disc diffusion test and dupleks polymerase chain reaction in Jambi city hospitals

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) remains the important health problems because the frequency of isolate MRSA tends to increase in the world. Cefoxitin disc diffusion test and detection of MecA gene using PCR were used in MRSA identification. FemB gen is used for spesies SA confirmation. FemB encodes enzyme in cross-linking peptidoglycan in SA. This research aimed to identified MRSA from clinical samples of hospitalized patients in Jambi city using cefoxitin disc diffusion test and dupleks PCR. This research has been conducted in Biomedic Laboratory of Medical Faculty of Jambi University during periode June – November 2017. Swab of 76 festering wounds were grown on Mannitol Salt Agar medium which incubate at 30ºC 18–24 h. The Yellowish colony confirmed with gram staining and tested using Staphytect plus Test DR 850 M. Identified of MRSA using cefoxitin disc diffusion test and dupleks PCR. This research reported cefoxitin-RSA was detected in 41,67% of SA, MecA gene was detected in 45,83% of SA. MecA gene was detected in 100% of cefoxitin-RSA and 7,14% of cefoxitin-SSA. FemB gene was detected in 8,33% of cefoxitin-RSA, and 64,28% of cefoxitin-SSA. Detection of MecA and FemB genes by PCR for identification of MRSA is sensitif and fast.