Abstract
Summary: We have developed an efficient method to isolate the male gametic nuclei(generative nuclei)from pollen grains of Lilium longiflorum and analyzed the chromatin proteins. As a result, it was revealed that the generative nuclei contained three kinds of specific histone variants(p22. 5, p21, p18. 5)in addition to five kinds of somatic histones. Immunofluorescence staining using specific antisera clearly demonstrated that these histone variants were specific to male gametic(generative and sperm)nuclei in L. longiflorum. Because the deduced amino acid sequences of the cDNAs of these specific variants showed some homology to somatic histones H2B, H3and H2A, respectively, they were designated gH2B, gH3and gH2A. They are the first male gamete-specific histones in angiosperms, and are assumed to be related to the condensation of chromatin of the male gametic nuclei.
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