Electron microscopic ammonical silver reaction forTradescantia pollen grain

Abstract

The postformalin ammonical silver reaction (ASR) for basic nuclear proteins was applied to both the generative and vegetative nuclei ofTradescantia pollen grains and examined with the electron microscope. The ASR deposit was usually observed mainly in the dense chromatic regions of both the generative and vegetative nuclei. Since a large amount of dense chromatic regions was observed in the generative nucleus than the vegetative ones in late or mature pollen grains, as a results, the increased amount of the ASR deposit was revealed in the generative nucleus.