Abstract
Aim:The study aimed to detect the invA gene in Salmonella isolated from milkfish in the Sidoarjo wet fish market.Materials and Methods:A total of 84 samples were prepared in enrichment media and isolated on the surface of Salmonella Shigella Agar. Salmonella growth produces transparent colonies with blackish color in the middle due to H2S gas formation. Samples were identified as Salmonella based on macroscopic colony morphology. Presumptive Salmonella sp. was put on Bismuth Sulfite Agar media. Salmonella was determined based on the results of the biochemical test that has been carried out using Microbact identification kits from negative gram staining.Results:The results of this study indicate that 32 of 84 samples (38.09%) were Salmonella bacteria. Furthermore, the invA gene detection was carried out using the polymerase chain reaction technique. Electrophoresis results showed four positive samples contained invA gene with a length of 284 bp.Conclusion:Results in this study indicate that contamination of milkfish with Salmonella needs strict hygienic measures to prevent their transmission to human.
Highlights
Indonesia is an agricultural country that has a large enough fishery potential
The invA gene detection was carried out using the polymerase chain reaction technique
Results in this study indicate that contamination of milkfish with Salmonella needs strict hygienic measures to prevent their transmission to human
Summary
Indonesia is an agricultural country that has a large enough fishery potential. The amount of capture fisheries production in 2014 was 6.20 tons, an increase of 0.34 million tons from 2013 which was only 5.86 million tons. The amount of fish production from the aquaculture sector has increased from year to year [1]. Some fish products contaminated with bacteria can cause a disease called foodborne disease. Foodborne disease or called foodborne diarrheal disease is a zoonotic disease and is found throughout the world. Foodborne diarrheal disease is a disease transmitted by animal’s carriers that are healthy to humans through food contamination [2]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
