Abstract
5 PRRSV antigen has also been observed in the Kupffer cells of hepatic sinusoids and in macrophages in the thymus, renal interstitium, and adrenal gland. 7 The cell type infected by PRRSV in these tissues has been identified primarily by cell morphology because of difficulties with staining for specific surface cell markers in situ. A flow cytometric (FACS) protocol for the simultaneous staining and evaluating of cell surface markers and intracellular viral proteins has been described for the study of human immunodeficiency virus (HIV). 13 Such a method would
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