Detection of Immunoglobulin E with a Graphene-Based Field-Effect Transistor Aptasensor

Yi Lan,Mitra Dutta,Min Choi,Ke Xu,Peter Burke,Sidra Farid,Yung Yu Wang,Saadia Ranginwala,Xenia Meshik,Michael A Stroscio

doi:10.1155/2018/3019259

Yi Lan, Mitra Dutta + Show 8 more

Open Access

https://doi.org/10.1155/2018/3019259

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Abstract

DNA aptamers have the ability to bind to target molecules with high selectivity and therefore have a wide range of clinical applications. Herein, a graphene substrate functionalized with a DNA aptamer is used to sense immunoglobulin E. The graphene serves as the conductive substrate in this field-effect-transistor-like (FET-like) structure. A voltage probe in an electrolyte is used to sense the presence of IgE as a result of the changes in the charge distribution that occur when an IgE molecule binds to the IgE DNA-based aptamer. Because IgE is an antibody associated with allergic reactions and immune deficiency-related diseases, its detection is of utmost importance for biomedical applications.

Highlights

Allergic reactions and allergic conditions have a significant impact on a person’s health that have become the leading cause of chronic illness globally
We have successfully demonstrated detection of K+ and Pb2+ ions, cytokines such as interferon-gamma (IFN-γ), and adenosine monophosphate (AMP) with graphene-based platforms [11,12,13]
The immunoglobulin E (IgE) aptamer is attached onto the graphene surface, and 5 μL of 1 μM IgE solution is added in turn into the well containing phosphatebuffered saline (PBS) while keeping VDS constant at 0.3 V

Summary

Introduction

Allergic reactions and allergic conditions have a significant impact on a person’s health that have become the leading cause of chronic illness globally. Allergic triggers may include food, insect stings, environmental factors, chemicals, or a condition of strong family history of atopy; the response often includes production of an immunoglobulin E (IgE) antibody [3]. These antibodies are a major component of the body’s immune system that can help fight antigens like viruses, bacteria, and fungi that are believed to be harmful to the body [4]. Their ease of modification, enhanced chemical and thermal stability, and ability to denaturalize under unfavorable conditions make them a viable candidate for biomedical sensing applications [8, 9]

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