Detection of HPV and MMTV in breast cancer cases

Abstract

Objective To analyse the presence of human papillomavirus and mouse mammary tumor virus in breast cancer, and expected to provide a possible target for gene therapy of breast cancer. Methods PCR and nested PCR assay were used to detect the HPV L1, HPV16E6, HPV16E7, HPV18E6, HPV18E7 and the enevelop gene of MMTV, while sequence analysis was performed in 76 paraffin embedded tissues of breast cancer. Results The percentage of HPV L1, HPV18 E6, HPV18 E7 and the enevelop gene of MMTV was repectively 9.21%, 6.58%, 9.21%and 30.26%, while no HPV16 E6 and HPV16E7 was found in 76 paraffin embedded tissues of breast cancer. Conclusions HPV18 and the enevelop gene of MMTV was existed in breast cancer, there was low connection between HPV and breast cancer, but there may be some correlation between MMTV and breast cancer. This research offers a possible target for gene therapy of breast cancer. Key words: Papillomavirus, human; Mammary tumor virus, mouse; Breast neoplasms; Polymerase chain reaction