Detection of high-risk human papillomavirus types-16 and-18 in laryngeal squamous cell carcinoma

Abstract

Objective To investigate the relationship between human papilloma virus(HPV)infection and the development of laryngeal squamous cell carcinoma(LSCC)． Methods To elucidate the role of HPV in the development of LSCC，we employed polymerase chain reaction(PCR)based on four pairs of primers an4 in situ hybridization(ISH)to screen the HPV infection in 84 ISCC tissues．Results Using HPV L1 general primer amplification，HPV DNA was detected in 23(27．4％)of the 84 LSCC samples．However，when specific primers for HPV-16 or-18 were used to amplify E6 and E7 in all samples，29 cases(34．5％)were positive for HPV-16，while 6 cases(7．1％)were positive for HPV 18．Coinfeetion of HPV-16 and-18 were found in 4cases (4．8％)．Overall，HPV type 16 and 18 infections were present in 36．9％ of the LSCC samples．In addition，the positive rate of HPV 16 E6 mRNA was 30．9％(26/84)in LSCC by ISH with digoxin—labeled sense probes of HPV 16 E6．Conclusion High—risk HPV-16may be an etiologic factor in the development of laryngeal squamous cell carcinoma, while the complicated molecular mechanism of HPV16 inducedtumorgenesis needs a further study． Key words: Human papillomavirus; Laryngeal neoplasms; Polymerase chain reaction; In situ hybridization