Detection of expressing of miR-103/KLF4 in A549 lung cancer cells and A549/DDP resistant cells and validation of the targeted relationship

Abstract

Objective To study the expression and targeted relationship of miR-103/KLF4 (Kruppel like factor) in A549 and resistant cell lines of lung cancer. Methods To culture the A549 cell lines and A549/DDP (cisplatin) resistant cell lines in vitro and detect survival rates by methyl thiazolyl tetrazolium (MTT) method. Real-time polymerase chain reaction (RT-PCR) and Western blotting were used to test the expression of miR-103/KLF4 of these cell lines. Dual-luciferase reporter gene experiment to detect the targeted relationship. Results A higher expression of miR-103 and a obvious lower expression of KLF4 were observed in A549/DDP resistant cell lines. MiR-103 target KLF4-3′ UTR (3′untranslated regions) directly in lung cancer cells. Conclusions In A549/DDP resistant cells, miR-103 can regulate KLF4 at target. Key words: MicroRNAs/ME; Kruppel-like transcription factors/ME; Lung neoplasms/PA/ME; Drug resistance, neoplasm