Abstract
Detection of European Strain of<i>Echinococcus multilocularis</i>in North America
Highlights
Two independent subsamples of cyst material were fixed in 70% ethanol and shipped to the University of Regina, Regina, Saskatchewan, for PCR using primers targeting microsatellite loci EmsB and NAK 1 [5,6]
The British Columbia (BC) dog sample was homozygous for the 198-bp allele at NAK 1, a genotype found in a variety of locations in Europe and Japan but not in North America, where the dominant genotype appears to be homozygous 192 [5]
Mitochondrial and microsatellite characterization showed that the genotype of E. multilocularis found in the BC dog was most similar to those described from west-central Europe
Summary
Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCRamplified DNA of the gene encoding the protein rOmpA. Address for correspondence: José de la Fuente, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; email: jose_delafuente@yahoo.com Detection of European Strain of Echinococcus multilocularis in North America
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