Abstract
The usage of microbiological screening tests is widespread in control of presence of antimicrobial drug residues in meat samples. Screening tests must be capable to detect antimicrobial drug residue of interest and detection limits must comply with MRL (Maximum Residue Limit). The aim of this study was to examine the performance of a microbiological screening test with E. coli as test microorganism: capability of detecting enrofloxacina and it?s main metabolite ciprofloxacine at MRL levels in both fortified and incurred chicken tissue samples. Detection limits of microbiological screening test with E. coli was 50 ng/g for enrofloxacin and 25 ng/g for ciprofloxacin. Screening test had positive results in all samples of fortified and incurred meat with residue concentrations above MRL level. The results of this examinations shows that microbiological screening test with E. coli, as simple and cost effective test, is capable to detect enrofloxacine and it?s metabolite ciprofloxacine in treated poultry at MRL level ie test is capable to detect unsafe poultry meat.
Highlights
Antimicrobial drug treatment is widespread in food producing animals breeding
The aim of this study was to examine the performance of screening test microbiological method with E. coli as test microorganism: capability to detect enrofloxacin and ciprofloxacin at MRL levels in both fortified and incurred chicken tissue samples
In a view of antimicrobial residue detection in food, a positive sample is the sample that contains residues at level above the MRL. This value is the basic parameter for sample assessment, since samples containing residues below MRL level are considered negative, i.e. safe
Summary
Meat for human consumption should be safe i.e. residues of antimicrobial drugs in meat should be below MRL (Maximum Residue Limit). Fluoroqunolone antimicrobial drugs are semi-synthetic antimicrobial agents. Fluoroquinolonesa are used both in human and veterinary medicine. Enrofloxacin is fluoroquinolone antimicrobial developed exclusively for the use in veterinary medicine. Intensive poultry production, housing systems, management practices have influence on poultry health (Lolly et al, 2013). Common poultry infections, such as mycoplasmal infections, colibacilosis and pasteurelosis, frequently are treated with enrofloxacin (Martinez et al, 2006). Ciprofloxacin is the main methabolite of enrofloxacin and has bactericidal activity, as parent compound is approved in human medicine
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
