Abstract
In order to identify and develop the specific DNA marker for the identification of Hanwoo (Korean Cattle) from other breeds, a specific DNA marker of 519 bp was identified and sequenced from polymorphic analysis using RAPD-PCR for 6 cattle breeds. Two different repetitive sequences, (AAC)5 and (GAAGA)2, were selected and designed to use specific probe to develop a DNA marker for Hanwoo specific. When the (AAC)5 probe was applied, the 10 kb specific DNA marker showed in the DNA fingerprinting from 237 of 281 Hanwoo individuals. This novel Hanwoo specific DNA probe is useful to perform the marker-assisted selection for screening Hanwoo purity as an unique genetic source. (Asian-Aust. J. Anim. Sci. 2002. Vol 15, No. 8 : 1071-1075)
Highlights
YEO ET AL.DNA cloning and sequencingThe DNA was extracted from a gel slice using the JET sorb kit (GENOMED Inc., NC, USA) and amplified again by PCR reaction with the same primer
DNA markers to distinguish Hanwoo from five foreign marker for Hanwoo was observed in two individuals of cattle breeds
We found that a DNA band of 519 bp from RAPD-PCR
Summary
ABSTRACT : In order to identify and develop the specific DNA marker for the identification of Hanwoo (Korean Cattle) from other breeds, a specific DNA marker of 519 bp was identified and sequenced from polymorphic analysis using RAPD-PCR for 6 cattle breeds. The number of plant and animal species, the major consequence ease with which many RAPD polymorphic loci can be being a loss of global genetic diversity requires sets of identified without primer sequence information, and the genetic markers that characterize distinct populations. Differentiation of Hanwoo cattle, which represent valuable This local breed has been threatened with animal genetic resources in Korea. Two. A recent technique capable of detecting DNA Asian breeds were Japanese Black and Chinese Yanbian polymorphism is based on random amplification by the which have the same origin as Hanwoo.
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