Abstract
Multiplex polymerase chain reaction (mPCR) technique without DNA extraction was developed for detection of pathogenic bacteria in frozen minced pork. Five primer pairs were designed for amplification of target DNA fragments of 5 bacterial species namely Campylobacter fetus , Escherichia coli , Listeria monocytogenes , Salmonella enterica serovar Typhi and Vibrio cholerae . The results showed that the new method of multiplex PCR technique in this study was specific to the target bacteria with sensitivity at 10 4 cells/g.
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