Abstract
Ice cubes are a complement to drinks. Ice cubes must not contain Coliform or Escherichia coli. Coliform and Escherichia coli are indicator microorganisms used in water quality analysis. Its presence indicates fecal contamination. The purpose of this study was to analyze the presence of Coliform and E.coli on ice cubes found in drinks sold around the Kampus 4 Universitas Ahmad Dahlan. Sampling was done by purposive sampling. The sample used in this study was 7 ice cube samples taken from different stalls around the campus. This study used the MPN (Most Probable Number) method which consists of a presumptive test, a confirmative test and the Escherichia coli test. A positive result was indicated by changes in the turbidity of the media and the presence of gas bubbles in the durham tube. Data analysis was performed by comparing the number of positive tubes in the observation results with the MPN table. Based on the data obtained, samples of ice cubes from 7 sellers around Kampus 4 Universitas Ahmad Dahlan were positive for Coliform and Escherichia coli bacteria. The principles of food sanitation hygiene need to be improved to prevent bacterial contamination on ice cubes.
Highlights
Ahmad Dahlan were positive for Coliform and E.coli bacteria
Uji Kualitas Air Sungai Rengganis dan Pantai Timur Pangandaran dari CemaranMetamorfosa: Journal Of Biological Sciences, 4(2), 224-230. doi:10.24843/metamorfosa.2017.v04.i02. p14
Summary
Hasil positif ditandai dengan perubahan kekeruhan media LB dan adanya gelembung gas pada tabung durham. Hasil positif ditandai dengan perubahan kekeruhan media BGLB dan adanya gelembung gas pada tabung durham (Wulansari et al, 2017; Romesberg dan Henderson, 2018). Hasil positif ditandai dengan perubahan kekeruhan media ECB dan adanya gelembung gas pada tabung durham (Romesberg dan Henderson, 2018). Hasil positif dari uji pendugaan ditandai dengan terbentuknya gelembung gas dan berubahnya kekeruhan media (Gambar 1a). Lactose broth dan Lauril Tryptose Broth dapat digunakan sebagai media pada deteksi Coliform, tetapi banyak bakteri lain juga memfermentasi laktosa sehingga semua tabung positif pada uji pendugaan harus dilanjutkan dengan uji penegasan (Rompré et al, 2002). Bakteri Coliform yang tumbuh pada media ini akan ditandai dengan perubahan media menjadi keruh dan adanya gelembung gas pada tabung durham (Gambar 1b). Hasil yang telah diperoleh kemudian dibandingkan dengan tabel MPN untuk mendapatkan nilai indeks MPN
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