Abstract
The essential oil extracted from the oregano (Origanum vulgare) is rich in phenolic compounds that have strong antioxidant and antibacterial properties. Carvacrol is the major component of oregano essential oil. The current study describes the use of a molecularly imprinted quartz crystal microbalance (QCM) sensor to detect carvacrol in oregano essential oil. For the formation of the sensing film, the surface of a quartz crystal with a silver electrode was modified by simple drop coating with a synthesized polymer solution and thermally polymerized at 95°C in an inert atmosphere. The polymer was synthesized by copolymerization of styrene, methyl methacrylate, acrylonitrile and divinylbenzene in an optimum ratio with template carvacrol. The analytical performance of the sensor has been observed to be reasonably good (sensitivity – 1.24Hz/ppm) and has adequate repeatability and reproducibility. Linear calibration curves were obtained in the concentration range of carvacrol between 5–300 ppm. The molecularly imprinted polymer structure and surface morphology were analyzed by FTIR spectroscopy, SEM and AFM. Validation of the proposed sensor with oregano essential oil samples has been performed by correlating the frequency deviation of the sensor with the estimations of carvacrol obtained from gas chromatography.
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