Abstract
IntroductionThe carbapenem inactivation method (CIM) is a cost-effective assay for detecting carbapenemases. However, its interpretation is unclear for Pseudomonas spp. We evaluate its accuracy when meropenem is changed to imipenem. MethodsWe analyzed 266 P. aeruginosa isolates. The CIM method consists of: resuspend bacterial colonies (a full 10μL loop) in 400μL water, in which a 10μg disk of meropenem/imipenem is immersed. After 2h of incubation (35°C), remove the disk, place it onto a Mueller-Hinton agar plate previously inoculated with Escherichia coli (ATCC 25922), and incubate at 35 ̊C between 18-24 h. Interpretation criteria (mm of inhibition zone): ≤19mm, positive; ≥25mm negative; 20–24mm, undetermined. ResultsImipenem improves the sensitivity and specificity of CIM when compared to meropenem (99.4% and 98.9%, vs. 91.9% and 94.7%, respectively). ConclusionsThe accuracy of CIM for carbapenemase detection in P. aeruginosa is increased with the use of imipenem.
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