Detection of biofilm formation among the mastitis isolates of Staphylococci by evaluation of three different screening methods

Abstract

Internalization and biofilm formation is an important step in staphylococcal mastitis pathogenesis . Twenty five staphylococcal isolates of coagulase negative and coagulase positive were collected from clinical mastitis in cows, and biofilm production were studied for these isolate by three different methods. Tube method , tissue culture plate and Congo red agar method. The first two methods were used to study effect of glucose addition as source of polysaccharides that important in biofilm formation. The results showed significant increase in biofilm formation by addition of glucose in staphylococci coagulase negative diagnosed by tissue culture plate method. Congo red agar method results showed increase number of positive isolates by increasing incubation time. The results showed that tissue culture plate method (Spectrophotometric method) was the best method for the diagnosis of biofilm formation in comparison with both tube method and Congo red agar method.