Detection of Beta-lactamase Resistance in Klebsiella pneumoniae Isolated from Different Clinical Sources

Abstract

186 samples were collected from various pathogenic specimens, including 75 urine samples, 46 cerebrospinal fluid samples, 23 sputum samples, 9 body fluid samples, 14 blood samples, 4 discharge samples, 11 fluid samples from wounds and burns, and 4 bone marrow samples. Accordingly, 23 isolates of K. pneumoniae were identified as follows: 12 from urine samples, three isolates from cerebrospinal fluid, two each from catheters, wounds, and sputum, and one isolate from burns and bronchitis. The 23 isolates under study were tested for their susceptibility to 11 penicillin, cephalosporin, and carbapenem antibiotics, including beta-lactam and other antibiotics. The results showed high resistance of K. pneumoniae to piperacillin at a rate of 91%, and then to ampicillin at a rate of 86.9%, while were sensitive to both imipenem and meropenem, at a rate of 78%. The results showed that 100% of the isolates showed their ability to produce beta-lactamase enzymes as confirmed by the acid and mineral methods. The high percentage of enzymatic production indicates the presence of enzymatic resistance among bacterial isolates under study, which is why the bacteria gave high resistance to antibiotics. The results of the study showed the ability of 14 isolates to produce broad-spectrum beta-lactamase enzymes at a rate of 60%.