Detection of bacterial species from clinical mastitis in dairy cows at Nilphamari district and their antibiogram studies

Md Khaled Hossain,Md Shahin Mahmud,Md Shajedur Rahman,Md Titon Mia,Monoranjan Das,Nazmi Ara Rumi

doi:10.3329/ajmbr.v2i4.31011

Abstract

The present study was conducted on the rural dairy cows to detect the bacterial species from clinical mastitis in dairy cows with their antibiogram studies during the period from January 2015 to June 2015. For this purpose two upazilla were selected under the Nilphamari district. On the basis of morphology, staining, cultural and biochemical characteristics, the isolated organisms were classified as, Staphylococcus spp., Streptococcus spp., E. coli, and Bacillus spp. .For this study, a total of 48 samples were collected from affected mastitis cows. Out of 48 milk samples, 17 were positive for Staphylococcus spp. (35.42%), 9 were positive for Streptococcus spp. (18.75%), 7 were positive for E. coli (14.58%), 5 were positive for Bacillus spp.(10.41%),5 were positive for mixed organisms (10.41%) and 5 were unidentified organisms (10.41%). Antibiogram studies revealed that all of the isolates of Staphylococcus spp. were sensitive to gentamicin and were resistant to streptomycin. Gentamicin was sensitive to all of the isolates of Streptococcus spp. and was resistant to streptomycin. The isolates of Bacillus spp. were sensitive to ciprofloxacin and Bacillus spp. was resistant to streptomycin. All of the isolates of E. coli were sensitive to ciprofloxacin the isolates were resistant to ampicillin and amoxicillin. Over all sensitivity revealed that ciprofloxacin, gentamicin and enrofloxacin were most efficacious. Thus, it may be recommended that ciprofloxacin, gentamicin and enrofloxacin in optimum doses would resolve most cases of clinical mastitis in dairy cows.Asian J. Med. Biol. Res. December 2016, 2(4): 656-663

Highlights

Milk is a highly nutritious food that is rich in carbohydrate, proteins, fats, vitamins and minerals
Isolation and identification of bacterial species Isolation of bacterial flora directly from apparently clinically mastitis affected cows was identified as Staphylococcus spp
Among 48 milk samples, 17 were Staphylococcus spp. (35.41%), 9 were Streptococcus spp.(18.75%), 7 were E. coli (14.58%), 5 were Bacillus spp. (10.41%), 5 were mixed organisms (10.41%), and 5 were unidentified organisms (10.41%) and Cultural examination expressed that Staphylococcus spp.(35.41%) to be predominant organisms followed by Streptococcus spp. (18.75%), E. coli (14.58%) and Bacillus spp. (10.48%).Our findings are in agreement with findings of Abdel-Radyand Sayed (2009) in which they isolated Staphylococcus spp., bacillus spp.. and Escherichia coli from the positive mastitis samples with prevalence 52.5%, 31.5% and 16.25% respectively

Summary

Objectives

The objectives of this study are to isolate and identify the causative bacterial species of mastitis in dairy cows at Nilphamari district with their antibiogram profiles in the study area

Methods

Results

Discussion

Conclusion