Detection of antibiotics resistance e-coli gens isolates from urinary tract infection

Abstract

This study was completed in laboratories of Biology Department in Faculty of Science. It explains of antibiotics resistance Escherichia coli that isolated from urinary tract Infection patients in the province of Najaf. A total number of (225) samples were collected from patients with from hospitals and laboratories AL-Najaf Governorate, E.coli isolated from urinary tract Infection infection revealed that 80 E.coli isolates against 22 commonly used antibacterial agents by using the disk diffusion method. E. coli isolates has a great resistance to most commonly antibiotics used in treatment of urinary tract Infection, the highest rate of resistance is seen with, Ciprofloxacin, Levofloxacin 60 /80 ( 75 %) followed by Norfloxacine 57/80 (71.25 %) Trimethoprime-sulfamethaxazol 55/80 (68.75 %), The results showed that the( gyrA) gene was detected in E. coli isolates, from the 30 (100%) all isolates of E. coli were have (gyr A) gene. The results also showed that the (Sul1)gene was detected in E. coli isolates, from the 30 (100%) isolates of E. coli 24 (80%) were have (Sul1) gene, while the results showed four positive isolates of the Ant(2")-1a , from the 30 (100%) isolates of E. coli 4 (13.3%) were have ( Ant(2")-1a) gene.