Abstract

The development of a bio-assay for the detection of 4-quinolone residues in rainbow trout ( Oncorhynchus mykiss Walbaum) muscle is described. The bio-assay depends upon a particularly sensitive isolate of Yersinia ruckeri (Fish Diseases Laboratory, 39/81, Weymouth; National Collections of Industrial and Marine Bacteria, 13282, Aberdeen) allowing detection of residues down to 0.02 μg/ml in muscle extract. Traditional problems associated with bio-assays such as accuracy and precision were examined and potential cross-reaction with other antibiotics licensed for use in aquaculture with could affect the assay was also investigated. Alongside existing physicochemical techniques the bio-assay potentially provides an additional method both for quantifying residue levels in pharmacokinetic studies and for carcass screening prior to human consumption.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call