Abstract
In this paper, carbon quantum dot-labelled β-lactoglobulin antibodies were used for refractive index magnification, and β-lactoglobulin was detected by angle spectroscopy. In this method, the detection light is provided by a He-Ne laser whose central wavelength is the same as that of the porous silicon microcavity device, and the light source was changed to a parallel beam to illuminate the porous silicon microcavity’ surface by collimating beam expansion, and the reflected light was received on the porous silicon microcavity’ surface by a detector. The angle corresponding to the smallest luminous intensity before and after the onset of immune response was measured by a detector for different concentrations of β-lactoglobulin antigen and carbon quantum dot-labelled β-lactoglobulin antibodies, and the relationship between the variation in angle before and after the immune response was obtained for different concentrations of the β-lactoglobulin antigen. The results of the experiment present that the angle variations changed linearly with increasing β-lactoglobulin antigen concentration before and after the immune response. The limit of detection of β-lactoglobulin by this method was 0.73 μg/L, indicating that the method can be used to detect β-lactoglobulin quickly and conveniently at low cost.
Highlights
In this paper, carbon quantum dot-labelled β-lactoglobulin antibodies were used for refractive index magnification, and β-lactoglobulin was detected by angle spectroscopy
The immunoreactivity of the β-LG antigen and Carbon quantum dots (CQDs)-labelled β-LG antibody at different concentrations was tested by an angle spectrum
CQD-labelled β-LG antibody at different concentrations was tested by an angle spectrum assay applying a spectroscopy-free device
Summary
Carbon quantum dot-labelled β-lactoglobulin antibodies were used for refractive index magnification, and β-lactoglobulin was detected by angle spectroscopy. In this method, the detection light is provided by a He-Ne laser whose central wavelength is the same as that of the porous silicon microcavity device, and the light source was changed to a parallel beam to illuminate the porous silicon microcavity’ surface by collimating beam expansion, and the reflected light was received on the porous silicon microcavity’ surface by a detector. The main allergen in milk is bovine β-LG, which is a foreign protein acquired by infants and young children. PSi sensors are classified into two main types: PSi chemical sensors [9] and PSi optical sensors [10]
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