Abstract
A reversed-phase ion-pairing high-performance liquid chromatography method for the detection and separation of SAM486A in human plasma and urine is described. Precipitation of proteins was used for plasma sample preparation. Enrichment of SAM486A on a 5 μ C18 column using 0.05 M NaH 2PO 4 and 0.005 M pentan-sulfonic acid (pH 3.0) as eluent was followed by isocratic elution onto a 5 μ C18 analytical column using 0.01 M NaH 2PO 4 and 0.005 M pentan-sulfonic acid (pH 3.0) as eluent. Analysis time was 23.0 ± 0.1 min. The separation parameters were: capacacity factor = 6.21; plates/m = 15,002; peak tailing = 2.076. The method is linear between 5 ng/ml (detection limit) and 1000 ng/ml.
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